Hairless 마우스 조직에서의 저온 상압 플라즈마의 안정성 평가
Evaluation of the safety of non-thermal atmospheric-pressure plasma in hairless mouse tissues
박상례, 김규천, 최별보라, 김지영,
소속 상세정보
박상례 ( Park Sang-Rye ) - 경남정보대학교 치위생과
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학전문대학원 구강해부학교실
최별보라 ( Choi Byul-Bo-Ra ) - 동서대학교 치위생학과
김지영 ( Kim Ji-Young ) - 경남정보대학교 치위생과
KMID : 0355420140380030148
Abstract
Objectives: The aim of the present study was to evaluate the stability of non-thermal atmospheric-pressure plasma on Candida albicans in hairless mouse-2 (HRM-2) tissues.
Methods: HRM-2 mice were subjected to non-thermal atmospheric-pressure plasma jet treatment us-ing an optical fiber probe and monitored using a thermometer. The skin of HRM-2 mice was treated with plasma jet for 0, 60, 180, and 300 s per day for 5 days. After plasma treatment, morphological changes in Candida albicans on the skin of these mice were examined using a scanning electron microscope. Biopsy of the plasma-treated skin was performed and the tissues were histologically analyzed using he-matoxylin and eosin (H&E) and Masson’s trichrome stains.
Results: The scanning electron microscopic images revealed the morphological changes in the mem-brane structure of the plasma-treated Candida albicans. Histological analysis showed that non-thermal plasma treatment did not cause epidermal damage or tissue inflammation and did not significantly modify the collagen layers of the mouse skin.
Conclusions: The results of this study suggest that non-thermal atmospheric-pressure plasma might be safe and effective for clinical applications in the field of dentistry.
키워드
Candida albicans;Hematoxylin and eosin stain;Masson’s trichrome stain;Non-thermal atmospheric pressure plasma;Scanning electron microscopy
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